Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)) Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)) Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)) Growth promotion test in accordance with the harmonised method of EP, USP and JP. Typical composition (g/litre): Pepton from casein 17.0 Pepton from soymeal 3.0 D(+)-Glucose monohydrate 2.5 Sodium chloride 5.0 di-Potassium hydrogen phosphate 2.5. Stability test (7 days, room temperature) If halogenated, they should be collected in container B. 15 th Edition.Casein-peptone soymeal-peptone broth, Casein-peptone soyameal-peptone brothģ Relatively unreactive organic reagents should be collected in container A. Public Health Laboratory Service, London (1995).Ħ. Compendium of Methods for the Microbiological Examination of Foods. Alternatively, they should be prepared by incubation in TSB (casein soya bean digest broth) at 30-35☌ for 18-24 hours. Test strains are available in Quanti-Cult™ format. Inoculate portions of medium with not more than 100 CFU of the following micro-organisms and incubate at 30-35☌ for ≤3 days. Microbial Enumeration Tests (Growth promotion test and Test for suitability of count method in presence of product) Quality control for non-sterile pharmaceutical products †† after incubation at 20-25◦ C for 5 days † after incubation at 20-25◦ C for 3 days * this organism is available in Quanti-Cult™ format

White mycelia, with or without black spores † Inoculate portions of medium with not more than 100 cfu of the following micro-organisms: Organism

Incubate portions of medium at 20-25☌ for 14 days. Quality control for sterile pharmaceutical products * This organism is available as a Culti-Loop™ Quality control for non-pharmaceutical products Store the prepared medium away form the light at room temperature.ĭehydrated medium: Straw coloured, free-flowing powder Store the dehydrated medium at 10-30☌ and use before the expiry date on the label. Tryptone Soya Broth is used in food bacteriology as the basal medium to which a variety selective agents are added for selective enrichment of Staphylococcus aureus 2 and Escherichia coli O157 3. A positive result is revealed after 24-48 hours incubation at 55☌, by a heavy growth of organisms causing turbidity in the broth. Tryptone Soya Broth is recommended in the USP (Casein soya bean digest broth) for the recovery of organisms after sterilisation processes 1. Oxoid laboratory tests have shown that Tryptone Soya Broth has a greater ability to resuscitate heated spores of Geobacillus stearothermophilus than Dextrose Tryptone Broth. Tryptone Soya Broth is especially suitable for the tube dilution method of antibiotic susceptibility testing. Five to 10ml of blood may be added to 50ml of medium. Anticoagulants such as `liquid’† (sodium polyanethyl sulphonate) or sodium citrate may be added to the broth prior to sterilisation. The superior growth-promoting properties of Tryptone Soya Broth make it especially useful for the isolation of organisms from blood or other body fluids. For this purpose, the broth (with added agar) should be used soon after sterilisation, or, heated and cooled just before inoculation. The addition of a small amount of agar renders the broth suitable for the cultivation of obligatory anaerobes, such as Clostridium spp. Cultures should be examined at frequent intervals, as maximum growth is reached earlier than with less nutritious media and the phase of decline consequently begins sooner. Tryptone Soya Broth may be used for the cultivation of aerobes and facultative anaerobes, including some fungi. Tryptone Soya Broth conforms to formulations detailed in various international pharmacopoeias 1,4,5,6. Due to the inclusion of both Tryptone and Soya Peptone, the medium will support a luxuriant growth of many fastidious organisms without the addition of serum, etc. Sterilise by autoclaving at 121☌ for 15 minutes.Ī highly nutritious versatile medium which is recommended for general laboratory use. TRYPTONE SOYA BROTH (Casein soya bean digest medium) EP/USP/JP/BPĪ highly nutritious general purpose medium for the growth of bacteria and fungiĪdd 30g to 1 litre of water (purified as required), mix well and distribute into final containers.

Bacto tryptic soy broth